Testsealabs Chikungunya IgM Test

Chikungunya IgM Test

1. I-Target Analyte: Lokhu kuhlolwa kukhomba ngokuqondile amasosha omzimba ekilasi le-IgM akhiqizwa amasosha omzimba womuntu ukuphendula ukutheleleka ngegciwane leChikungunya. Amasosha omzimba e-IgM ngokuvamile awokuqala ukuvela ngesikhathi sokutheleleka okuyingozi, ngokuvamile abonakala phakathi kwezinsuku ezi-3-7 ngemva kokuqala kwezimpawu futhi aqhubeke amasonto ambalwa kuya ezinyangeni. Ngakho-ke ukutholakala kwabo kuyinkomba ebalulekile yokutheleleka kwe-CHIKV yakamuva noma eyingozi.
2. Ukuhambelana Kwesifanekiso: Ukuhlolwa kugunyazwe ukusetshenziswa nezinhlobo eziningi zamasampula, okunikeza ukuguquguquka kwezilungiselelo zokunakekelwa kwezempilo ezahlukahlukene:

• Igazi Eliphelele (Uthi Lomunwe noma I-Venipuncture): Inika amandla ukunakekelwa kwendawo ngokushesha noma ukuhlolwa okuseduze kwesiguli ngaphandle kwesidingo sokucubungula isampula eyinkimbinkimbi.
• I-Serum: Uhlobo olusagolide lwesampula lokutholwa kwe-antibody kuzilungiselelo zaselabhorethri.
• I-Plasma: Inikeza enye indlela ye-serum, evame ukutholakala kalula kumalebhu omtholampilo.

3. Ukusetshenziswa Okuhlosiwe & Inani Lokuxilonga: Inhloso eyinhloko yalolu hlolo ukusiza ochwepheshe bezokunakekelwa kwezempilo ekuxilongeni ukutheleleka kwegciwane le-Chikungunya acute. Umphumela omuhle we-IgM, ikakhulukazi uma uhlotshaniswa nezimpawu zomtholampilo (ukushisa okukhulu okungazelelwe, ubuhlungu obukhulu bamalunga, ukuqubuka, ikhanda elibuhlungu, njll.) kanye nomxholo we-epidemiological (ukuya noma ukuyohlala ezindaweni eziwumqedazwe), inikeza ubufakazi obunamandla obusekela ukutheleleka okusebenzayo noma kwakamuva kakhulu kwe-CHIKV. Kubaluleke kakhulu esigabeni sokuqala sokugula lapho amasosha omzimba e-IgG engase angatholakali.
4. Isimiso Sobuchwepheshe: Ngokusekelwe kubuchwepheshe bokugeleza kwe-chromatographic immunoassay:

• I-Colloidal Gold Conjugate: Umugqa wokuhlola uqukethe iphedi ene-antigen ye-CHIKV ehlanganiswe nezinhlayiya zegolide eli-colloidal.
• Ukugeleza Kwesampula: Uma isampula (igazi, i-serum, noma i-plasma) isetshenziswa, ithutha ngokwechromatographic eduze komugqa.
• I-Antibody Capture: Uma amasosha omzimba e-CHIKV-specific IgM ekhona kusampula, azobophezela kuma-antigen e-CHIKV ahlanganiswe negolide, enze i-antigen-antibody complex.
• Ukuthwebula Umugqa Wokuhlola: Le nkimbinkimbi iyaqhubeka nokugeleza futhi ithathwe amasosha omzimba e-IgM anganyakazi endaweni yomugqa Wokuhlola (T), okuholela emugqeni onombala obonakalayo.
• Ulayini Wokulawula: Ulayini Wokulawula (C), oqukethe amasosha omzimba abopha i-conjugate kungakhathaliseki ukuthi amasosha omzimba e-CHIKV, kufanele abonakale njalo ukuze aqinisekise ukuthi ukuhlola kusebenze kahle futhi isampuli ithuthe kahle.

5. Imiphumela Esheshayo: Ukuhlolwa kunikeza umphumela obonakalayo, wekhwalithi (Omuhle/Omubi) ngokuvamile phakathi kwemizuzu eyi-10-20, okusiza ukuthathwa kwezinqumo zomtholampilo ngokushesha.
6. Ukusebenzisa Kalula: Idizayinelwe ukwenza kube lula, edinga ukuqeqeshwa okuncane futhi azikho izisetshenziswa eziyisipesheli zokutolikwa kwemiphumela, ikwenze ifaneleke izilungiselelo ezihlukahlukene ezihlanganisa imitholampilo, amalabhorethri, kanye nokusetshenziswa kwenkundla okungenzeka kube khona ngesikhathi sokuqubuka kwezifo.
7. Okucatshangelwayo Okubalulekile:

• Ikhwalithi: Lokhu ukuhlolwa kokuhlola okunikeza impendulo ethi Yebo/Cha ngobukhona bamasosha omzimba e-IgM, hhayi inani (titer).
• Ukuxhumana Komtholampilo: Imiphumela kufanele ihunyushwe ngokuhambisana nomlando womtholampilo wesiguli, izimpawu, ubungozi bokuchayeka, nokunye okutholwe elabhorethri. Amasosha omzimba e-IgM kwesinye isikhathi angaphikelela noma ahlangane namagciwane ahlobene (isb, i-O'nyong-nyong, i-Mayaro), okungenzeka aholele ekutholeni amanga. Ngokuphambene, ukuhlola kusenesikhathi kakhulu ekuthelelekeni (ngaphambi kokuba i-IgM ikhuphukele kumazinga aqaphelekayo) kungaveza amanga angamanga.
• Ukuhlola Okuhambisanayo: Kwamanye ama-algorithms okuxilonga, i-IgM ephozithivu ingase ilandelwe nezivivinyo eziqondile (njenge-Plaque Reduction Neutralization Test - PRNT) ukuze kuqinisekiswe, noma ukuhlolwa kwe-IgG okubhanqiwe (kumasampula abukhali kanye ne-convalescent) kungase kusetshenziselwe ukubonisa ukuguqulwa kwe-seroconversion.