ʻO Testsealabs Chikungunya IgM hōʻike

Ho'āʻo Chikungunya IgM

1. Analyte Target: Hoʻomaopopo pono kēia hoʻāʻo ʻana i nā antibodies papa IgM i hana ʻia e ka ʻōnaehana pale kanaka i ka pane ʻana i ka maʻi maʻi Chikungunya. ʻO nā antibodies IgM ka mea maʻamau ka mea mua e ʻike ʻia i ka wā o kahi maʻi maʻi, ʻike pinepine ʻia i loko o 3-7 mau lā ma hope o ka hoʻomaka ʻana o nā hōʻailona a hoʻomau i kekahi mau pule a i nā mahina. No laila ʻo kā lākou ʻike ʻana he hōʻailona koʻikoʻi o ka maʻi CHIKV hou a i ʻole maʻi.
2. Mea Hoʻohālikelike: Hoʻopaʻa ʻia ka hoʻāʻo no ka hoʻohana ʻana me nā ʻano laʻana he nui, e hāʻawi ana i ka maʻalahi no nā ʻano mālama olakino like ʻole:

• ʻO ke koko holoʻokoʻa (Fingerstick a i ʻole Venipuncture): Hiki i ka hoʻāʻo wikiwiki ʻana a i ʻole ka hoʻāʻo kokoke i ka maʻi me ka ʻole o ka hoʻoili ʻana i ka laʻana.
• Serum: ʻO ke ʻano hoʻohālike gula maʻamau no ka ʻike ʻana i ka antibody i nā hoʻonohonoho keʻena.
• Plasma: Hāʻawi i kahi ʻokoʻa i ka serum, hiki ke loaʻa maʻalahi i nā keʻena lapaʻau.

3. Hoʻohana ʻia a me ka waiwai diagnostic: ʻO ke kumu nui o kēia hoʻāʻo ʻana ʻo ke kōkua ʻana i nā limahana mālama ola i ka ʻike ʻana i ka maʻi maʻi maʻi Chikungunya. ʻO kahi hopena IgM maikaʻi, ʻoi loa ke hoʻopili ʻia me nā hōʻailona lapaʻau (ka wela kiʻekiʻe koke, ka ʻeha ʻeha nui, ka ʻeha, ka ʻeha ʻeha, a me nā mea ʻē aʻe) a me ka pōʻaiapili epidemiological (huakaʻi a i ʻole ka noho ʻana ma nā wahi endemic), hāʻawi i nā hōʻike kākoʻo ikaika no ka maʻi CHIKV ikaika a i ʻole hou loa. He waiwai nui ia i ka wā mua o ka maʻi inā ʻaʻole ʻike ʻia nā antibodies IgG.
4. Manaʻo ʻenehana: Ma muli o ka ʻenehana immunoassay chromatographic kahe ʻaoʻao:

• Colloidal Gold Conjugate: Aia i loko o ka pahu ho'āʻo kahi pad me CHIKV antigen i hui pū ʻia me nā ʻāpana gula colloidal.
• Kahe Laʻana: Ke hoʻohana ʻia ka hāpana (ke koko, serum, a i ʻole ka plasma), neʻe ia ma ka chromatographically ma ke kaula.
• Hopu Antibody: Inā loaʻa nā antibodies IgM kikoʻī CHIKV i ka hāpana, e hoʻopaʻa lākou i nā antigens CHIKV i hoʻohui ʻia i ke gula, e hana ana i kahi paʻakikī antigen-antibody.
• Hoʻopaʻa Laina Hoʻāʻo: Ke kahe mau nei kēia paʻakikī a hopu ʻia e nā antibodies IgM anti-human immobilized ma ka ʻāpana laina ʻo Test (T), i hopena i kahi laina kala ʻike ʻia.
• Laina Manaʻo: Pono e ʻike mau ʻia kahi laina Mana (C), i loaʻa nā antibodies e hoʻopaʻa i ka conjugate me ka nānā ʻole ʻana i nā antibodies CHIKV, pono e hōʻoia i ka holo pono ʻana o ka hoʻāʻo a ua neʻe pono ka hāpana.

5. Nā hualoaʻa wikiwiki: Hāʻawi ka hōʻike i kahi hopena hiʻona, kūpono (Positive/Negative) maʻamau i loko o 10-20 mau minuke, e hoʻomaʻamaʻa i ka hoʻoholo wikiwiki.
6. ʻO ka maʻalahi o ka hoʻohana ʻana: Hoʻolālā ʻia no ka maʻalahi, koi ʻia ka liʻiliʻi o ka hoʻomaʻamaʻa ʻana a ʻaʻohe mea hana kūikawā no ka wehewehe ʻana i ka hopena, kūpono ia no nā ʻano hoʻonohonoho like ʻole me nā keʻena lapaʻau, laboratories, a me ka hoʻohana ʻana i ke kula i ka wā o nā maʻi ʻino.
7. Nā manaʻo nui:

• Kūlana: He hōʻike hōʻike kēia e hāʻawi ana i ka pane ʻAe/ʻAʻole no ka loaʻa ʻana o nā antibodies IgM, ʻaʻole ka nui (titer).
• Hoʻoponopono Pilikino: Pono e unuhi ʻia nā hopena me ka mōʻaukala lapaʻau o ka mea maʻi, nā hōʻailona, ​​​​ka pilikia o ka ʻike, a me nā ʻike ʻē aʻe. Hiki i nā antibodies IgM i kekahi manawa ke hoʻomau a hoʻololi paha me nā maʻi e pili ana (e laʻa, O'nyong-nyong, Mayaro), hiki ke alakaʻi i nā hopena maikaʻi ʻole. ʻO ka mea ʻē aʻe, ʻo ka hoʻāʻo ʻana i ka maʻi ma mua (ma mua o ka piʻi ʻana o IgM i nā pae hiki ke ʻike ʻia) hiki ke hoʻopuka i nā mea ʻino.
• Ho'āʻo Complementary: Ma kekahi mau algorithm diagnostic, hiki ke hahai ʻia kahi IgM maikaʻi me nā hoʻokolohua kikoʻī hou aʻe (e like me ka Plaque Reduction Neutralization Test - PRNT) no ka hōʻoia ʻana, a i ʻole ka hoʻāʻo ʻana IgG i hoʻopaʻa ʻia (ma nā laʻana acute a convalescent) e hōʻike i ka seroconversion.