Ikizamini Indwara Ikizamini Malariya Ag pf / pv Ikizamini cya Tri-umurongo

Kwipimisha Indwara Ikizamini Malariya Ag pf / pv Ikizamini cya Tri-Line Ikigereranyo cyerekanwe

Ibisobanuro bigufi:

Malariya Ag Pf / Pv Tri-umurongo Ikizamini Cassette ni immunoassay yihuta ya chromatografique kugirango hamenyekane ubuziranenge bwa plasmodium falciparum histidinerich protein-II (HRP-II) na plasmodiumvivax lactate.dehydrogenase (LDH) mumaraso yose kugirango ifashe mugupima malariya (Pf / Pv).

Ibisubizo Byihuse: Laboratoire-Nukuri muminota	Laboratoire-Icyiciro Cyuzuye: Yizewe & Yizewe
Ikizamini Ahantu hose: Nta laboratoire isabwa	Ubwiza bwemewe: 13485, CE, Mdsap Yubahiriza
Byoroheje & Byoroheje: Byoroshye-Gukoresha, Zeru Hassle	Amahirwe meza: Gerageza neza murugo

Ohereza imeri kuri twe Kuramo nka PDF

Ibicuruzwa birambuye

Ibicuruzwa

Ibicuruzwa birambuye:

Ubwoko bw'icyitegererezo:
- Amaraso yose (urutoki cyangwa venipuncture yamaraso).
Igihe cyo Kumenya:
- Iminota 15-20(ibisubizo bigomba gusobanurwa muminota 20; ibisubizo nyuma yiki gihe bitemewe).
Ibyiyumvo byihariye:
- Ibyiyumvo:Mubisanzwe> 90% mugutahura indwara zombi za Pf na Pv.
- Umwihariko:Mubisanzwe> 95% kuri Pf na Pv byombi.
Uburyo bwo kubika:
- Ubike hagati4 ° C na 30 ° C., kure y'izuba ritaziguye.
- Ntukonje.
- Ubuzima bwa Shelf busanzwe buvaAmezi 12 kugeza 24, ukurikije amabwiriza yabakozwe.
Ibisobanuro Ibisobanuro:
- Igisubizo cyiza:
  - Imirongo itatu iragaragara:
    1. C (Igenzura) umurongo(yerekana ikizamini gifite ishingiro).
    2. Umurongo wa Pf(niba hagaragaye antigen ya Plasmodium falciparum).
    3. Umurongo wa Pv(niba hagaragaye antigens ya Plasmodium vivax).
    - Kuba hari imirongo ya Pf na / cyangwa Pv byerekana kwandura amoko ya malariya.

Ihame:

Immunochromatographic Assay:
Cassette yikizamini irimo ubudahangarwaantibodies za monoclonalyihariye antigens ya Plasmodium (urugero,HRP-2kuri Pf napLDHkuri Pv).

Iyo amaraso ashyizwe mubizamini, nibamalariya antigenszirahari, zizahuza na antibodiyite ya zahabu muri sample, izagenda ikurikirana ikizamini cya capillary.
NibaPlasmodium falciparumantigen iramenyekana, umurongo wamabara uzakora kuriUmurongo wa Pf.
NibaPlasmodium vivaxantigen iramenyekana, umurongo wamabara uzakora kuriUmurongo wa Pv.
UwitekaUmurongo wo kugenzura (C)iremeza ko ikizamini gikora neza kandi cyerekana agaciro k'ikizamini.

Ibigize:

Ibigize	Umubare	Ibisobanuro
IFU	1	/
Ikizamini	25	Buri mufuka wifunze urimo igikoresho kimwe cyo gupima hamwe na desiccant
Gukuramo	500μL * 1 Tube * 25	Tris-Cl buffer, NaCl, NP 40, ProClin 300
Inama	1	/
Swab	/	/

Uburyo bw'ikizamini:


1. Karaba intoki zawe	2. Reba ibikubiye mubikoresho mbere yo kwipimisha, shyiramo pake, cassette yikizamini, buffer, swab.	3. Shyira umuyoboro wo gukuramo mu kazi.	4.Kuraho kashe ya aluminiyumu kuva hejuru yumuyoboro ukuramo urimo buffer.

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5.Kwitonda witonze ukureho swab udakoze ku isonga. Shyiramo isonga yose ya swab cm 2 kugeza kuri 3 mumazuru yiburyo. Andika aho kumena izuru. Urashobora kubyumva ukoresheje intoki zawe mugihe winjizamo izuru cyangwa ukabigenzura muri mimnor. Siga imbere yizuru mumuzenguruko inshuro 5 byibuze amasegonda 15, Noneho fata izuru rimwe hanyuma uzinjize mu zindi zuru. Shyira imbere yizuru mumuzenguruko inshuro 5 byibuze amasegonda 15. Nyamuneka kora ikizamini muburyo butaziguye kandi ntukore
kureka guhagarara.

6. Shyira swab mu muyoboro wo gukuramo. Ongera uhindure swab mu masegonda agera ku 10, Hinduranya swab kurwanya umuyoboro ukuramo, ukande umutwe wa swab imbere imbere yigituba mugihe ukanda impande zumuyoboro kugirango urekure amazi menshi ashoboka muri swab.


7. Kuramo swab muri paki udakora kuri padi.	8. Vanga neza ukanda hasi yigituba. Shyira ibitonyanga 3 byicyitegererezo uhagaritse kurugero rwicyitegererezo cya cassette. Soma ibisubizo nyuma yiminota 15. Icyitonderwa: Soma ibisubizo muminota 20. Ubundi, ni ugusaba ikizamini birasabwa.

Ibisubizo Ibisobanuro: