Ikizamini cya Chikungunya IgM Ikizamini

Chikungunya IgM Ikizamini

1. Intego Analyte: Iki kizamini kigaragaza neza antibodi zo mu rwego rwa IgM zakozwe na sisitemu y’umubiri y’umuntu mu rwego rwo guhangana na virusi ya Chikungunya. Antibodiyite za IgM mubusanzwe nizo zambere zigaragara mugihe cyanduye gikaze, mubisanzwe zishobora kugaragara mugihe cyiminsi 3-7 nyuma yuko ibimenyetso bitangiye kandi bikomeza ibyumweru byinshi ukwezi. Kumenya kwabo rero nikimenyetso gikomeye cyubwandu bwa vuba cyangwa bukabije CHIKV.
2. Kugereranya Ibigereranyo: Ikizamini cyemewe gukoreshwa hamwe nubwoko butandukanye bw'icyitegererezo, gitanga ihinduka ryimiterere itandukanye yubuzima:

• Amaraso Yose (Fingerstick cyangwa Venipuncture): Ifasha byihuse-by-ubuvuzi cyangwa kwipimisha hafi y’abarwayi bidakenewe gutunganywa byoroshye.
• Serumu: Ubwoko bwa zahabu bwicyitegererezo cyo kumenya antibody muri laboratoire.
• Plasma: Tanga ubundi buryo bwa serumu, akenshi biboneka muri laboratoire.

3. Guteganya Gukoresha & Gusuzuma Agaciro: Intego yibanze yiki kizamini ni ugufasha inzobere mu buvuzi mu gusuzuma virusi ikaze ya Chikungunya. Igisubizo cyiza cya IgM, cyane cyane iyo gifitanye isano nibimenyetso byubuvuzi (umuriro mwinshi utunguranye, ububabare bukabije bwo mu ngingo, guhubuka, kubabara umutwe, nibindi) hamwe nindwara ya epidemiologiya (gutembera cyangwa gutura ahantu h’icyorezo), itanga ibimenyetso bifatika byerekana kwandura indwara ya CHIKV cyangwa vuba aha. Ifite agaciro cyane mugice cyambere cyindwara mugihe antibodies za IgG zishobora kutamenyekana.
4. Ihame rya tekinoloji: Ukurikije urujya n'uruza rwa chromatografique immunoassay tekinoroji:

• Ihuriro rya Zahabu ya Koleji: Igice cyo kwipimisha kirimo padi hamwe na antigen ya CHIKV ihujwe na zahabu ya colloidal.
• Icyitegererezo Cyuzuye: Iyo icyitegererezo (maraso, serumu, cyangwa plasma) gishyizwe mubikorwa, cyimuka muburyo bwa chromatografique kumurongo.
• Ifatwa rya Antibody: Niba antibodiyite ya IgIK yihariye ya IgM ihari murugero, izahuza na antigene ya CHIKV ya zahabu, ikore antigen-antibody.
• Gufata Umurongo Wibizamini: Uru ruganda rukomeje gutemba kandi rufatwa na antibodiyide zirwanya abantu IgM zidahagarikwa mukarere ka Line (T), bikavamo umurongo wamabara ugaragara.
• Umurongo wo kugenzura: Umurongo wo kugenzura (C), urimo antibodies zihuza conjugate utitaye kuri antibodiyite za CHIKV, ugomba guhora ugaragara kugirango wemeze ko ikizamini cyakoze neza kandi icyitegererezo cyimutse neza.

5. Ibisubizo Byihuse: Ikizamini gitanga ibisubizo bigaragara, byujuje ubuziranenge (Ibyiza / Ibibi) mubisanzwe muminota 10-20, byorohereza gufata ibyemezo byihuse.
6. Kuborohereza gukoreshwa: Byashizweho kubworoshye, bisaba amahugurwa make kandi nta gikoresho cyihariye cyo gusobanura ibisubizo, bigatuma gikwira ahantu hatandukanye harimo amavuriro, laboratoire, hamwe nogukoresha umurima mugihe cyaduka.
7. Ibitekerezo by'ingenzi:

• Ubwiza: Iki nikizamini cyo gusuzuma gitanga Yego / Oya igisubizo kuberako antibodiyite za IgM, ntabwo ari ingano (titer).
• Isano rya Clinical: Ibisubizo bigomba gusobanurwa bifatanije namateka yubuvuzi bwumurwayi, ibimenyetso, ibyago byo guhura nabyo, nibindi byagaragaye muri laboratoire. Antibodiyite za IgM zirashobora rimwe na rimwe gutsimbarara cyangwa guhuza virusi zifitanye isano (urugero, O'nyong-nyong, Mayaro), bishobora kuganisha ku byiza bibi. Ibinyuranye, kwipimisha hakiri kare kwandura (mbere yuko IgM izamuka kugera kurwego rushobora kugaragara) birashobora gutanga ingaruka mbi.
• Kwipimisha Byuzuye: Muri algorithms zimwe na zimwe zo gusuzuma, IgM nziza irashobora gukurikiranwa hamwe nibizamini byihariye (nka Plaque Reduction Neutralisation Test - PRNT) kugirango byemezwe, cyangwa ibizamini bya IgG byombi (ku byitegererezo bikaze kandi bihamye) bishobora gukoreshwa kugirango berekane serokonversion.