Testsealabs Chikungunya IgM Test

Chikungunya IgM Test

1. Target Analyte: Muedzo uyu unonyatso ratidza IgM class masoja ekudzivirira chirwere anogadzirwa neanhu immune system mukupindura hutachiona hweChikungunya. Masoja ekudzivirira chirwere eIgM ndiwo anowanzo tanga kuoneka panguva yechirwere chetachiona, chinowanzoonekwa mukati memazuva 3-7 mushure mekutanga kwezviratidzo uye kuramba kwemavhiki akati wandei kusvika kumwedzi. Kuonekwa kwavo saka chiratidzo chakakosha chechangobva kuitika kana chakanyanya CHIKV hutachiona.
2. Specimen Kuenderana: Muedzo wakatenderwa kuti ushandiswe neakawanda sampuli mhando, ichipa kuchinjika kune akasiyana ehutano marongero:

• Ropa Rakazara (Fingerstick kana Venipuncture): Inogonesa kukurumidza-yekutarisira-yekutarisira kana pedyo-yemurwere kuongororwa pasina kudiwa kwezvakaoma sampuli kugadzirisa.
• Serum: Iyo yegoridhe yakajairwa sampuli mhando yekuonekwa kweanorwisa utachiona mumarabhoritari marongero.
• Plasma: Inopa imwe nzira kune serum, inowanzowanikwa nyore mumakiriniki labs.

3. Kushandiswa Kwakanangana & Kuongorora Kukosha: Chinangwa chikuru chebvunzo iyi kubatsira vashandi vehutano mukuongororwa kwehutachiona hweChikungunya virus. Mhedzisiro yakanaka yeIgM, kunyanya kana yakabatana nezviratidzo zvekiriniki (fivha yakanyanya, kurwadziwa kwakanyanya kwemajoini, kuputika, musoro, etc.) uye epidemiological mamiriro (kufamba kuenda kana kugara munzvimbo dzine magumo), inopa humbowo hunotsigira hwakasimba hwehutachiona hunoshanda kana huchangobva kuitika hweCHIKV. Iyo inonyanya kukosha muchikamu chekutanga chechirwere apo maIgG ​​antibodies angave asati aonekwa.
4. Technology Nheyo: Kubva pane lateral kuyerera chromatographic immunoassay tekinoroji:

• Colloidal Goridhe Conjugate: Mutambo wekuyedza une padhi ine CHIKV antigen yakasanganiswa kune colloidal goridhe zvimedu.
• Kuyerera Kwemuenzaniso: Kana sampuro (ropa, serum, kana plasma) ikaiswa, inotama chromatographically parutivi rwemutsetse.
• Antibody Capture: Kana CHIKV-specific IgM antibodies aripo mumuenzaniso, anosunga kune goridhe-conjugated CHIKV maantigen, achigadzira antigen-antibody complex.
• Test Line Capture: Iyi yakaoma inoramba ichiyerera uye inobatwa neanorwisa-munhu IgM masoja ekudzivirira ekudzivirira padunhu reTest (T) mutsara, zvichikonzera mutsara unooneka weruvara.
• Kudzora Mutsara: Mutsara (C) mutsara, une ma antibodies anosunga conjugate pasinei neCHIKV antibodies, inofanira kugara ichionekwa kuti inosimbisa muedzo wakashanda zvakanaka uye sampuli yakatama zvakanaka.

5. Kurumidza Mibairo: Muedzo unopa inooneka, yemhando mhedzisiro (Yakanaka/Negative) kazhinji mukati me10-20 maminetsi, kufambisa sarudzo yekiriniki nekukurumidza.
6. Kureruka Kwemashandisirwo: Yakagadzirirwa kuve nyore, inoda kudzidziswa kushoma uye isina yakasarudzika chiridzwa chekududzira mhedzisiro, ichiita kuti ive yakakodzera kune akasiyana marongero anosanganisira makiriniki, marabhoritari, uye ingangove inoshandiswa mumunda panguva yekubuda.
7. Zvinokosha Zvinotarisirwa:

• Hunhu: Iyi bvunzo yekuongorora inopa Hongu/Kwete mhinduro yekuvapo kweIgM masoja ekudzivirira chirwere, kwete huwandu (titer).
• Clinical Correlation: Mhedzisiro inofanirwa kududzirwa pamwe chete nenhoroondo yekiriniki yemurwere, zviratidzo, njodzi yekufumurwa, uye zvimwe zvakawanikwa murabhoritari. Masoja ekudzivirira chirwere eIgM dzimwe nguva anogona kuramba kana kupindirana nemavhairasi ane hukama (semuenzaniso, O'nyong-nyong, Mayaro), zvichizotungamira kune manyepo. Zvikasadaro, kuyedza kukasira hutachiona (IgM isati yakwira kusvika pamazinga anooneka) inogona kuburitsa zvisirizvo.
• Completement Testing: Mune mamwe diagnostic algorithms, yakanaka IgM inogona kuteverwa nemimwe bvunzo (sePlaque Reduction Neutralization Test - PRNT) yekusimbisa, kana paired IgG bvunzo (paacute uye convalescent samples) inogona kushandiswa kuratidza seroconversion.