Testsealabs Chikungunya IgM Test

Kev kuaj IgM

1. Lub Hom Phiaj Analyte: Qhov kev sim no tshwj xeeb txheeb xyuas IgM cov tshuaj tiv thaiv kab mob uas tsim los ntawm tib neeg lub cev tiv thaiv kab mob hauv kev teb rau tus kab mob Chikungunya. IgM cov tshuaj tiv thaiv feem ntau yog thawj zaug tshwm sim thaum muaj tus kab mob hnyav, feem ntau tuaj yeem kuaj pom tsis pub dhau 3-7 hnub tom qab pib cov tsos mob thiab pheej mus ob peb lub lis piam mus rau lub hlis. Lawv qhov kev kuaj pom yog li ntawd ib qho tseem ceeb qhia tau hais tias tsis ntev los no los yog mob CHIKV kab mob.
2. Specimen Compatibility: Qhov kev ntsuam xyuas tau siv tau nrog ntau hom qauv, muab kev yooj yim rau kev kho mob sib txawv:

• Cov Ntshav Tag Nrho (Ntiv tes lossis Venipuncture): Ua kom muaj kev saib xyuas sai sai lossis nyob ze-tus neeg mob kuaj yam tsis tas yuav tsum tau ua cov qauv ua haujlwm nyuaj.
• Serum: Cov qauv qauv kub rau kev kuaj xyuas cov tshuaj tiv thaiv hauv cov chaw kuaj mob.
• Plasma: Muaj lwm txoj hauv kev rau ntshav qab zib, feem ntau yooj yim muaj nyob rau hauv cov chaw kuaj mob.

3. Npaj Siv & Diagnostic Tus Nqi: Lub hom phiaj tseem ceeb ntawm qhov kev sim no yog los pab cov kws kho mob hauv kev kuaj mob tus kab mob Chikungunya tus kab mob. Qhov txiaj ntsig zoo ntawm IgM, tshwj xeeb tshaj yog thaum muaj kev cuam tshuam nrog cov tsos mob tshwm sim (ua npaws siab, mob pob qij txha hnyav, pob khaus, mob taub hau, thiab lwm yam) thiab cov ntsiab lus sib kis (mus ncig lossis chaw nyob hauv thaj chaw muaj kab mob), muab pov thawj muaj zog txhawb rau tus kab mob CHIKV nquag lossis tsis ntev los no. Nws yog qhov tseem ceeb tshwj xeeb tshaj yog nyob rau theem pib ntawm tus mob thaum IgG cov tshuaj tiv thaiv tseem tsis tau kuaj pom.
4. Tswv yim Technology: Raws li lateral flow chromatographic immunoassay technology:

• Colloidal Kub Conjugate: Cov kab ntawv xeem muaj ib lub ncoo nrog CHIKV antigen conjugated rau colloidal kub hais.
• Sample Flow: Thaum cov qauv (ntshav, ntshav, los yog ntshav) yog siv, nws migrate chromatographically raws lub strip.
• Antibody Capture: Yog hais tias CHIKV-specific IgM cov tshuaj tiv thaiv muaj nyob hauv cov qauv, lawv yuav khi rau kub-conjugated CHIKV antigens, tsim cov tshuaj tiv thaiv kab mob antigen.
• Test Line Capture: Qhov nyuaj no txuas ntxiv mus thiab raug ntes los ntawm kev tiv thaiv tib neeg IgM cov tshuaj tiv thaiv immobilized ntawm thaj tsam Test (T) kab, uas ua rau pom cov xim muaj xim.
• Tswj Kab: Ib kab tswj (C), muaj cov tshuaj tiv thaiv kab mob uas khi cov kab mob tsis hais txog CHIKV cov tshuaj tiv thaiv kab mob, yuav tsum nco ntsoov tshwm sim kom paub meej tias qhov kev sim tau ua haujlwm raug thiab cov qauv tau tsiv teb tsaws zoo.

5. Cov txiaj ntsig nrawm: Qhov kev ntsuam xyuas muab qhov pom, qhov txiaj ntsig zoo (zoo / tsis zoo) feem ntau hauv 10-20 feeb, ua kom yooj yim rau kev txiav txim siab sai.
6. Kev Siv Yooj Yim: Tsim los rau kev yooj yim, yuav tsum tau muaj kev cob qhia tsawg thiab tsis muaj cov cuab yeej tshwj xeeb rau kev txhais cov txiaj ntsig, ua rau nws tsim nyog rau ntau qhov chaw suav nrog cov chaw kho mob, chaw kuaj mob, thiab muaj peev xwm siv tau thaum muaj kev sib kis.
7. Cov ntsiab lus tseem ceeb:

• Kev Tsim Nyog: Qhov no yog qhov kev tshuaj ntsuam xyuas muab cov lus teb Yog / Tsis yog rau qhov muaj cov tshuaj tiv thaiv IgM, tsis yog qhov ntau (titer).
• Clinical Correlation: Cov txiaj ntsig yuav tsum tau muab txhais ua ke nrog tus neeg mob keeb kwm kho mob, cov tsos mob, kev pheej hmoo raug kis, thiab lwm yam kev kuaj pom. IgM cov tshuaj tiv thaiv qee zaum tuaj yeem tshwm sim lossis cuam tshuam nrog cov kab mob cuam tshuam (xws li O'nyong-nyong, Mayaro), uas ua rau muaj qhov tsis zoo. Hloov pauv, kev sim ntxov dhau ntawm tus kab mob (ua ntej IgM tau nce mus rau qib kuaj tau) tuaj yeem ua rau muaj qhov tsis zoo.
• Kev Ntsuam Xyuas Ntxiv: Hauv qee qhov kev kuaj mob algorithms, qhov zoo IgM tuaj yeem ua raws nrog cov kev sim tshwj xeeb (xws li Plaque Reduction Neutralization Test - PRNT) rau kev pom zoo, lossis ua ke IgG kuaj (ntawm cov qauv mob hnyav thiab convalescent) tuaj yeem siv los ua qauv seroconversion.