SARS-COV-2 Kubogamiye AntiBy Gutahura Kit (Elisa)
【Gukoresha】
SARS-COV-2 Kubogamiye AntiBy AntiBys AntiBy ni ENDYNME-Lyzyme-Lusa) yagenewe kumenya neza Antibodies zose zidafite aho zibogamiye kuri antibodied-cov-2 muri simune y'abantu na plasma. Ibihe- COV-2 Kutabogama Ibikoresho bya Antibods Gutahura Birashobora gukoreshwa nkimfashanyo mukumenya abantu bafite ubudahangarwa budakizwa Ibihe bya Sars-cov-2 Kutabogama AntiBy Ibikoresho bya Antibods ntibigomba gukoreshwa mugusuzuma ibitego bikaze-COV-2.
【Intangiriro】
Indwara ya coronavirus mubisanzwe bitera kutabogama ibisubizo bya antibody. Ibiciro SeroConvice muri Covid-19 ni 50% na 100% kumunsi wa 7 na 14 post onset,. Kugaragaza ubumenyi, virusi ijyanye no kutabogama antibody mumaraso ni intego yo kumenya kurwanya antibody hamwe nubushake bwo kutabogama antibody yerekana neza. Kugabanya ibibanza byo kugabanya ikizamini cyo kugaburira (PRNT) byamenyekanye nkibisanzwe bya zahabu yo kumenya antibodi. Ariko, kubera amafaranga make yabyo kandi asabwa cyane kubikorwa, prnt ntabwo ari ngirakamaro kubinini binini bya serodignose no gusuzuma urukingo. Ibihe bya Sars-cov-2 Kutabogama AntiBy Gutahura Kit ishingiye kuri Enzyme-Lyzyme-ihumure, rishobora kumenya antibody ya antibody mu buryo bwuzuye mu buryo bw'amaraso nk'uko byiciro byo kwibandaho.
【Uburyo bw'ikizamini】
1.Mu gitsina gutandukanya, aliquot 120μl yinzira ya Hace2-HRP.
2.Add 6 μl ya kalibrators, ingero zitazwi, kugenzura ubuziranenge muri buri muyoboro no kuvanga neza.
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3.Isahani ifite isahani hamwe na plaalr kandi inkubate kuri 37 ° C muminota 60.
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5.Tuza isahani kumpapuro kugirango ukureho amazi asigaye mumariba nyuma yo gukaraba intambwe.
6.Add 100 μl ya TMB Umuti Kuri buri karindwi hanyuma ushishikarire isahani mu mwijima kuri 20 - 25 ° C muminota 20.
7.Add 50 μl yo guhagarika igisubizo kuri buri kintu kugirango uhagarike reaction.
8.Gereya kwinjiza umusomyi wa microplate kuri 450 nm muminota 10 (630nm nkuko ibikoresho birasabwa kubikorwa byingenzi.
