Testsealabs Chikungunya IgM Test

Chikungunya IgM Test

1. Target Analyte: Mayesowa amazindikiritsa ma antibodies a gulu la IgM opangidwa ndi chitetezo cha mthupi cha munthu poyankha matenda a Chikungunya virus. Ma antibodies a IgM nthawi zambiri amakhala oyamba kuwonekera pakadwala matenda oopsa, nthawi zambiri amawonekera mkati mwa masiku 3-7 chiyambireni zizindikiro ndikupitilira kwa milungu ingapo mpaka miyezi. Kuzindikira kwawo ndi chizindikiro chofunikira cha matenda a CHIKV aposachedwa kapena pachimake.
2. Kugwirizana kwa Zitsanzo: Mayesowa amatsimikiziridwa kuti agwiritsidwe ntchito ndi mitundu ingapo ya zitsanzo, kupereka kusinthasintha kwamakonzedwe osiyanasiyana azaumoyo:

• Magazi Athunthu (Chala Chala kapena Venipuncture): Imathandiza kuti asamalidwe mwachangu kapena kuyezetsa pafupi ndi odwala popanda kufunikira kokonza zitsanzo zovuta.
• Seramu: Mtundu wachitsanzo wagolide wozindikirika ndi ma antibody m'ma labotale.
• Plasma: Imapereka njira ina yosinthira seramu, yomwe nthawi zambiri imapezeka m'ma laboratories azachipatala.

3. Kugwiritsa Ntchito Koyenera & Kuzindikira Kufunika Kwambiri: Cholinga chachikulu cha mayesowa ndikuthandizira akatswiri azachipatala kuti adziwe matenda a Chikungunya virus. Chotsatira chabwino cha IgM, makamaka pamene chikugwirizana ndi zizindikiro zachipatala (kutentha kwakukulu kwadzidzidzi, kupweteka kwapakati, kupweteka kwa mutu, kupweteka mutu, etc.) ndi zochitika za miliri (kuyenda kapena kukhala m'madera omwe ali ndi kachilomboka), zimapereka umboni wamphamvu wochirikiza matenda a CHIKV omwe akugwira ntchito kapena aposachedwapa. Ndiwofunika kwambiri kumayambiriro kwa matendawa pomwe ma antibodies a IgG sangawonekerebe.
4. Mfundo Zamakono: Kutengera ukadaulo wa lateral flow chromatographic immunoassay:

• Colloidal Gold Conjugate: Mzere woyesera uli ndi pad yokhala ndi antigen ya CHIKV yolumikizidwa ku tinthu tating'ono tagolide.
• Kuyenda kwa Zitsanzo: Pamene chitsanzo (mwazi, seramu, kapena madzi a m'magazi) agwiritsidwa ntchito, chimasuntha motsatira mzerewu.
• Antibody Capture: Ngati ma antibodies a CHIKV-specific IgM alipo mu zitsanzo, amamanga ma antigen a CHIKV opangidwa ndi golide, kupanga antigen-antibody complex.
• Kujambula Mzere Woyesera: Vutoli likupitilira kuyenda ndipo limagwidwa ndi ma anti-anthu a IgM osasunthika pagawo la mzere wa Test (T), zomwe zimapangitsa mzere wowoneka bwino.
• Mzere Wowongolera: Mzere wa Control (C), wokhala ndi ma antibodies omwe amamangiriza conjugate mosasamala kanthu za ma antibodies a CHIKV, ayenera kuwoneka nthawi zonse kuti atsimikizire kuti mayeserowo agwira ntchito bwino ndipo chitsanzocho chasamuka bwino.

5. Zotsatira Zachangu: Mayesowa amapereka zowoneka bwino, zowoneka bwino (Zabwino / Zoyipa) nthawi zambiri mkati mwa mphindi 10-20, zomwe zimathandizira kupanga chisankho mwachangu.
6. Kugwiritsa Ntchito Mosavuta: Zapangidwira kuti zikhale zosavuta, zomwe zimafuna kuphunzitsidwa pang'ono komanso palibe zida zapadera zomasulira zotsatira, zomwe zimapangitsa kuti zikhale zoyenera m'makonzedwe osiyanasiyana kuphatikizapo zipatala, malo opangira ma labotale, komanso kugwiritsidwa ntchito m'munda panthawi ya miliri.
7. Mfundo Zofunika:

• Mkhalidwe: Uku ndi kuyesa kowunika komwe kumapereka yankho la Inde/Ayi la kupezeka kwa ma antibodies a IgM, osati kuchuluka (titer).
• Kulumikizana Kwachipatala: Zotsatira ziyenera kutanthauziridwa mogwirizana ndi mbiri yachipatala ya wodwalayo, zizindikiro zake, chiwopsezo chowonekera, ndi zina zomwe zapezeka mu labotale. Ma antibodies a IgM nthawi zina amatha kupitilirabe kapena kulumikizana ndi ma virus ogwirizana nawo (mwachitsanzo, O'nyong-nyong, Mayaro), zomwe zitha kubweretsa zotsatira zabodza. Mosiyana ndi zimenezi, kuyezetsa matenda atangoyamba kumene (IgM isanakwere kufika pamlingo wodziwika) kungapereke zolakwika zabodza.
• Kuyesa Kowonjezera: Mu njira zina zowunikira, IgM yabwino ikhoza kutsatiridwa ndi mayeso ena (monga Plaque Reduction Neutralization Test - PRNT) kuti atsimikizire, kapena kuyesa kwa IgG pawiri (pazitsanzo za pachimake ndi kutsitsimuka) kungagwiritsidwe ntchito kuwonetsa seroconversion.