Testsealabs SARS-CoV-2 Neutralizing Antibody Detection Kit (ELISA)

Takaitaccen Bayani:

 

Cassette na gwajin Neutralizing Antibody na SARS-CoV-2 shine saurin immunoassay na chromatographic. An tsara wannan tantancewar don gano ƙwararrun ƙwayoyin rigakafin ƙwayoyin cuta daga Cutar Coronavirus 2019. Ana iya amfani da shi tare da cikakken jinin ɗan adam, ruwan magani, ko samfuran plasma. Gwajin yana aiki azaman taimako don kimanta matakan rigakafin cutar coronavirus na ɗan adam wanda ke kawar da titers antibody.

 

gouSakamako cikin gaggawa: Lab-Madaidaici a cikin mintuna gouMatsakaicin darajar Lab: Abin dogaro & Amintacce
gouGwaji Ko'ina: Babu Ziyarar Lab da ake buƙata  gouIngantattun Ingancin: 13485, CE, Mai yarda da Mdsap
gouMai Sauƙi & Sauƙi: Sauƙi-da-Amfani, Hassle Sifili  gouMafi dacewa: Gwaji da kwanciyar hankali a Gida

Cikakken Bayani

Tags samfurin

KA'IDA

Kit ɗin Ganewar Ganewa na Tsabtace Tsakanin SARS-CoV-2 ya dogara ne akan tsarin ELISA gasa.

Yin amfani da yanki mai ɗaurin mai karɓa mai tsafta (RBD), furotin daga furotin mai saurin kamuwa da cuta (S) da tantanin halitta

mai karɓar ACE2, an ƙirƙiri wannan gwajin don yin kwaikwayi ma'amala mai hana ƙwayoyin cuta.

Calibrators, Ingantattun Gudanarwa, da samfuran jini ko samfuran plasma ana gauraye su da kyau a cikin dilution

buffer mai dauke da hACE2-HRP conjugate aliquoted a cikin kananan bututu. Sa'an nan kuma gauraye suna canjawa wuri a ciki

rijiyoyin microplate da ke dauke da recombinant recombinant SARS-CoV-2 RBD guntu (RBD) don

shiryawa. A lokacin shiryawa na mintuna 30, takamaiman rigakafin RBD a cikin calibrators, QC da

samfurori za su yi gasa tare da hACE2-HRP don ƙayyadaddun ɗaurin RBD da ba a iya motsi a cikin rijiyoyin. Bayan

da shiryawa, ana wanke rijiyoyin sau 4 don cire haɗin hACE2-HRP wanda ba a ɗaure ba. Magani na

Ana ƙara TMB kuma a sanya shi na tsawon minti 20 a cikin dakin da zafin jiki, yana haifar da ci gaban a

launin shuɗi. An dakatar da ci gaban launi tare da ƙari na 1N HCl, kuma abin sha shine

spectrophotometrically a 450 nm. Ƙarfin launi da aka kafa yana daidai da

adadin enzyme da ke nan, kuma yana da alaƙa da alaƙa da adadin ma'aunin da aka tantance ta hanya ɗaya.

Ta hanyar kwatanta tare da ma'auni na daidaitawa da aka samar ta hanyar calibrators da aka bayar, ƙaddamarwa na

neutralizing antibodies a cikin da ba a sani ba samfurin sai a lissafta.

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KAYAN DA AKE BUKATA AMMA BA'A SAMU BA

1. Ruwan da aka distilled ko datti

2. Madaidaicin pipettes: 10μL, 100μL, 200μL da 1 ml

3. Tukwici pipette da za a iya zubarwa

4. Microplate reader iya karanta absorbance a 450nm.

5. Takarda mai sha

6. Takardar hoto

7. Vortex mahautsini ko daidai

TATTAUNAWA DA MISALIN

1. Ana iya amfani da samfuran jini da Plasma da aka tattara a cikin bututu masu ɗauke da K2-EDTA don wannan kayan.

2. Ya kamata a rufe samfuran kuma ana iya adana su har zuwa awanni 48 a 2 ° C - 8 ° C kafin a tantance.

Samfuran da aka riƙe na tsawon lokaci (har zuwa watanni 6) yakamata a daskare su sau ɗaya kawai a -20 ° C kafin a tantance.

Guji maimaita daskarewa-narkewa.

PROTOCOL

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Shiri Reagent

1. Dole ne a fitar da duk reagents daga firiji kuma a bar su su koma cikin zafin jiki kafin amfani

(20 ° zuwa 25 ° C). Ajiye duk reagents a cikin firiji da sauri bayan amfani.

2. Duk samfurori da sarrafawa ya kamata a yi vortexed kafin amfani.

3. HACE2-HRP Magani Shiri: Tsarma hACE2-HRP maida hankali a 1: 51 dilution rabo tare da Dilution

Buffer. Misali, tsarma 100 μL na hACE2-HRP maida hankali tare da 5.0ml na HRP Dilution Buffer zuwa

Yi maganin hACE2-HRP.

4. 1× Wash Magani Shiri: Tsarma 20× Wash Magani da deionized ko distilled ruwa tare da

rabon girma na 1:19. Misali, tsoma 20 ml na Maganin Wanke 20×20 tare da 380 ml na deionized ko

distilled ruwa don yin 400 ml na 1 × Wash Magani.

Tsarin Gwaji

1. A cikin tubes daban-daban, aliquot 120μL na maganin hACE2-HRP da aka shirya.

2. Ƙara 6 μL na calibrators, samfurori da ba a sani ba, masu sarrafawa masu inganci a cikin kowane bututu da haɗuwa da kyau.

3. Canja wurin 100μL na kowane cakuda da aka shirya a cikin mataki na 2 a cikin rijiyoyin microplate daidai.

zuwa tsarin gwajin da aka riga aka tsara.

3. Rufe farantin tare da Plate Seler kuma a sanya shi a 37 ° C na minti 30.

4. Cire Plate Seler kuma a wanke farantin tare da kusan 300 μL na 1 × Wash Solution kowace rijiya har sau hudu.

5. Matsa farantin a kan tawul na takarda don cire ragowar ruwa a cikin rijiyoyin bayan matakan wankewa.

6. Ƙara 100 μL na Maganin TMB zuwa kowace rijiya kuma a saka farantin cikin duhu a 20 - 25 ° C na minti 20.

7. Ƙara 50 μL na Maganin Tsaya zuwa kowace rijiya don dakatar da amsawa.

8. Karanta absorbance a cikin microplate reader a 450 nm a cikin minti 10 (630nm kamar yadda m.

an ba da shawarar don mafi girman aiki daidai).

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