MonkeyPox Virus (mpv) nuclec nucleic acid polowe
Chiyambi
Kit imagwiritsidwa ntchito pozindikira za Vitro Oyenererana ndi kachilombo ka MonkeyPox Virus (MPV), milandu yosiyanasiyana ndi milandu yomwe ikufunika kuti ipangidwe matenda a MonkeyPOX Virust.
Kit imagwiritsidwa ntchito kudziwa mtundu wa F3l wa MPV ili pakhosi ndi amphuno a swab.
Zotsatira zoyeserera za izi ndizachipatala zokha ndipo siziyenera kugwiritsidwa ntchito ngati njira yokhayo yodziwira matenda. Ndikulimbikitsidwa kuwunika kwathunthu kwa chikhalidwe cha odwala
mawonetseredwe ndi mayeso ena a labotale.
Kugwiritsa Ntchito
| Mtundu wa Assay | swab swab ndi ma swab |
| Mtundu Woyeserera | Kalerombiri |
| Zoyeserera | Chisana |
| Kukula kwa mapaketi | 48Tests / 1 bokosi |
| Kutentha | 2-30 ℃ |
| Moyo wa alumali | Miyezi 10 |
Mawonekedwe a malonda
Maganizo
Izi zimapangitsa kuti mndandanda wa MPV F3l Jen ngati dera landamale. Nthawi yeniyeni ya ma enemarescence yochulukirapo katatu ndi ma eclecic acid mwachangu amagwiritsidwa ntchito kuwunika acid acid a ma eluresic acid chifukwa cha kusintha kwa zinthu. Dongosolo lonyansa limaphatikizapo kuwongolera kwamkati, komwe kumagwiritsidwa ntchito kuwunika ngati pali PCR inhitors mu zitsanzo kapena ngati maselo mu zitsanzo amatengedwa, omwe amatha kupewa vuto labodza.
Zigawo zazikulu
Chizindikiro chili ndi ma resugents pakukonzekera mayeso 48 kapena kuwongolera kwapadera, kuphatikizapo izi:
Reagent a
| Dzina | Zigawo zazikulu | Kuchuluka |
| Kuzindikira kwa MPV wobweretsera | Chibati yazomwezi ili ndi mg2 +, F3l Gene / Rnase P prober Probe, Buffer, Taq DNA enzyme. | Mayeso 48 |
WobweretseraB
| Dzina | Zigawo zazikulu | Kuchuluka |
| Mpv Kuwongolera koyenera | Okhala ndi chidutswa cha chandamale cha MPV | 1 chubu |
| Mpv Kuwongolera | Popanda chidutswa cha MPV | 1 chubu |
| DNA imasuta reagent | Kukonzanso komwe kuli tris, edta ndi triton. | 48pcs |
| Kuphatikizanso Konzanso | Madzi ochepera | 5m |
Chidziwitso: Zigawo za manambala osiyanasiyana sangathe kugwiritsidwa ntchito mosiyanasiyana
】Makhalidwe Alumali】
1.Tarter A / B ikhoza kusungidwa pa 2-30 ° C, ndipo moyo wa alumali ndi miyezi 10.
2. Phatitsani tsegulani chivundikiro choyesera pokhapokha mutakonzekera mayeso.
3.Mosagwiritsa ntchito machubu oyesa kupitirira tsiku lotha ntchito.
4.Mosagwiritsa ntchito chubu chotsitsa.
】Chida Chogwiritsidwa Ntchito】
Zoyenera kuyenera kwa LC480 PCR Systems, genier 48E zokha
】Zofunikira Zitsanzo】
Mitundu yosangalatsa: Throat Swabs zitsanzo.
2.Syampling yankho:Pambuyo kutsimikizira, ndikulimbikitsidwa kugwiritsa ntchito chubu wamba kapena kachilombo ka kachilombo kopangidwa ndi ma dinzhou dissesea biology la osonkhanitsa zitsanzo.
Throat Swab:Pukutani mabungwe a Phalateral Faryngeal Toyonis ndi khoma lopanda kumapeto kwake ndikuyika swab swablech, chotsani mchira, ndikulitsa chivundikiro cha chubu.
Kugawikira ndi Kutumiza:Zitsanzo kuti ziyesedwe ziyenera kuyesedwa posachedwa. Kutentha kwa mayendedwe kuyenera kusungidwa pa 2 ~ 8 ℃. Zitsanzo zomwe zitha kuyesedwa pa maola 24 zitha kusungidwa pa 2 ~ 8 ~ ndipo ngati zitsanzo sizingayesedwe mkati mwa maola 24, ziyenera kusungidwa mkati mwa maola 24, ziyenera kusungidwa mkati mwa maola 24 mpaka -70 ℃ (ngati palibe chosungira -70 ℃, itha kusungidwa ku -20 ℃ kwakanthawi), pewani kubwereza
kuzizira ndi kuwononga.
4.Popr zitsanzo zoperekera zitsanzo, kusungirako, ndi mayendedwe ndizovuta pa ntchitoyi.
】Njira Yoyesera】
1.Sample kukonza ndi zitsanzo zowonjezera
1.1 prempu
Pambuyo posakanikirana yankho pamwambapa ndi zitsanzo, tengani 30μl ya chitsanzo mu DNA imatulutsa chubu yokonzanso ndikusakaniza motere.
1.2 Kutsegula
Tengani 20μL yokonzanso zokonzanso ndikuwonjezera ku Regint yokonzanso, onjezerani 5μl yazomwe zili ndi ziwonetserozi) masekondi.
2. PCR Kukwezeka
2.1 Kwezani mbale yokonzedwa ndi PCR / machubu a fluoresiscence pcr, ulamuliro woyipa komanso kuwongolera koyenera kudzakhazikitsidwa pazoyeserera iliyonse.
2.2 ma fluorescent akhala:
1) Sankhani msewu wamtsogolo kwa MPV;
2) Sankhani hex / vic njira yamagetsi yoyang'anira mwayi;
Kusanthula kwa 3.Resluls
Khazikitsani mzere pamwamba pa malo owonera bwino kwambiri.
4.
4.1 Kuwongolera zoipa: Palibe mtengo wamtundu wa CT mu banja, Hex / Vic Channel, kapena CT> 40;
4.2 Kuwongolera koyenera: Mu banja, Hex / Vic Channel, CTP40;
4.3 Zofunikira pamwambazi ziyenera kukhuta mu kuyesa komweko, apo ayi zotsatira zoyipa ndizosavomerezeka ndipo kuyesaku kuyenera kubwerezedwanso.
】Dulani mtengo】
Sampuli imatengedwa ngati yabwino pamene: landamale ct≤40, kuwongolera kwamkati Ctp40.
】Kutanthauzira】
Kuwongolera kwapadera kwatha, ogwiritsa ntchito ayenera kuwona ngati pali chopondera pa Hex / Vic Channel, ngati kuli ndi ch40 Ogwiritsa ntchito amatha kupitiriza kusanthula kwake:
3.Fos zitsanzo ndi kuchuluka kwa gene wamkati kwalephera (Hex / Vic
Channel, CT> 40, kapena kuti palibe ma virus kapena otsika ma virus oletsa Vutol atha kukhala chifukwa cholephera, kuyeserera kuyenera kubwerezedwa kuchokera kuzolinga za fanizo;
4.Pakuti zitsanzo zabwino ndi kachilombo kake, zotsatira za ulamuliro wamkati sizikhudza;
Chifukwa cha zitsanzo zomwe zimayesedwa, kuwongolera kwamkati kumafunikira kuti zitheke mwanjira ina
Zidziwitso
Satifiketi yolemekezeka
Mbiri Yakampani
Ife, ma Dirzhou ayeso a biotechnology co., LTD ndi akatswiri othamanga omwe akukula mwachangu amafufuza, akupanga, kupanga ndi kugawa makanema am'madzi (IVD) kuyesa zachipatala.
Malo athu ndi GMP, Iso9001, ndi Iso13458 wotsimikizika ndipo tili ndi kuvomerezedwa ndi CE FDA. Tsopano tikuyembekezera mogwirizana ndi makampani owonjezera kuti mutuku mtima.
Timapanga mayeso a kubereka, mayesero matenda opatsirana, mayeso a mankhwala osokoneza bongo, mayeso am'mimba, mayeso a nyama ndi chitetezo amadziwika m'misika yakunyumba komanso yowonjezera. Zabwino kwambiri komanso zabwino zabwino zimatithandizira kuchita zopitilira 50%.
Njira Zopangira
1.preware
2.cover
3.cross membrane
4.Chitch
5.Sastsly
6.Panuko m'matumba
7.sed ukoko
8.Pake bokosi
9.Nnyvyment
Pewani tsoka latsopano: Konzani monga momwe MontYox imafalikira
Pa Ogasiti 14 Ili ndi nthawi yachiwiri yomwe yapereka chenjezo kwambiri lokhudza phokoso la MonkeyPox kuyambira pa Julayi 2022.
Pakadali pano, kufalikira kwa MonkeyPox kwafalikira kuchokera ku Africa kupita ku Europe ndi Asia, komwe kumakhala ndi milandu yodziwika ku Sweden ndi Pakistan.
Malinga ndi zomwe zaposachedwa kuchokera ku Africa CDC, chaka chino, mamembala 12 a ku Africa anena za milandu ya 18,737, kuphatikizapo milandu 3,601, ndi kufa kwa 2.89%.
01 Kodi MonkeyPax ndi chiyani?
MonkeyPox (MPX) ndi matenda owoneka bwino omwe amayambitsidwa ndi kachilombo ka MonkeyPox. Itha kupatsidwa kuchokera ku nyama kupita kwa anthu, komanso pakati pa anthu. Zizindikiro zofanana zimaphatikizapo kutentha thupi, zotupa, ndi lymphadenopathy.
Vutoli limalowa mu thupi la munthu kudzera mucous nembanemba ndi khungu losweka. Zoyambitsa matenda zimaphatikizapo milandu ya MonkeyPox ndi makola omwe ali ndi kachilombo, anyani, ndi ena omwe sianthu. Pambuyo podwala, nthawi ya makulitsidwe ndi masiku 5 mpaka 21, nthawi zambiri masiku 6 mpaka 13.
Ngakhale kuti anthu ambiri amagonjetsedwa ndi kachilombo ka monkeyPox, pali kutetezedwa kwina kwa MonkeyPax kwa iwo omwe atemera ka nthomba, chifukwa chofanana ndi ma virus ndi antigenic. Pakadali pano, monkeypox makamaka amafalikira pakati pa amuna omwe amagonana ndi amuna kudzera pakugonana, pomwe kubweretsa matenda kwa anthu onse kumakhala kotsika.
02 Kodi mukusintha bwanji?
Conder chaka chimodzi, matenda akuluakulu a kachilomboka, "Clade II," wachititsa kuti anthu ambiri ayambike padziko lonse lapansi. Ndili ndi nkhawa, kuchuluka kwa zochitika zomwe zimayambitsidwa ndi "dikirani," zomwe zimachitika kwambiri ndipo zimakhala ndi vuto lalikulu, likukula ndipo latsimikiziridwa kunja kwa dziko la Africa. Kuphatikiza apo, kuyambira pa Seputembara chaka chatha, chosokoneza chatsopano, chowopsa chololera chokhacho,Clade IB, "wayamba kufalikira mu Democratic Republic of the Congo.
Chinthu chodziwika bwino cha izi ndi chakuti azimayi ndi ana osakwana zaka 15 amakhudzidwa kwambiri.
Zambiri zikuwonetsa kuti zoposa 70% ya milandu ili ndi odwala osakwana zaka 15, ndipo pakati pa milandu, chiwerengerochi chimatuluka mpaka 85%. Makamaka,Mlingo wamaso kwa ana ndi wokwera kanayi kuposa akulu.
03 Kodi chiopsezo cha kubwereza kwa MonkeyPox ndi chiyani?
Chifukwa cha nyengo yokopa alendo komanso njira zoyendera padziko lonse lapansi, chiopsezo cha kufala kwa malire kwa Montersox. Komabe, kachilomboka makamaka kwa nthawi yayitali, monga kuchita zogonana, kukhudzana ndi khungu, komanso kupuma pang'ono kapena kuyankhulana ndi ena, kotero kuti munthu wofatsa ndi munthuyo amakhala wofooka.
04 Momwe Mungapewere MonkeyPAX?
Pewani kucheza ndi anthu omwe muli ndi vuto laumoyo. Apaulendo ayenera kulabadira kukwiya kwa MonkeyPox m'maiko awo komwe akupita ndikupewa kulumikizana ndi makoswe ndi anyani.
Ngati chiwopsezo chachikulu chimachitika, kumawunikira thanzi lanu masiku 21 ndipo pewani kulumikizana ndi ena. Ngati zizindikiro monga zotupa, matuza, kapena malungo akuwoneka, pitani kuchipatala mwachangu ndikudziwitsa dokotala.
Ngati wachibale kapena mnzake wapezeka ndi MonkeyPox, pewani zoteteza payekha, ndipo musakhudze zinthu, zofunda, zofunda, zofunda, ndi zinthu zina. Pewani kutsuka mabafa, ndipo nthawi zambiri muzitsuka ndi mpweya wabwino.
MonkeyPox Diagments
MonkeyPox Diagment Missicts imathandizira kutsimikizira matenda opatsirana pozindikira ma antigens kapena ma antibodies, omwe amathandizira mawonekedwe oyenera komanso othandizira, komanso amagwira ntchito yofunika kwambiri yolamulira matenda opatsirana. Pakadali pano, Ahui HuckBlue azachipembedzo Coulloglogy Co., Ltd. wapanga zojambula zotsatirazi:
MonkeyPox antigen mayeso a Kiti: Amagwiritsa ntchito njira yagolide yosungirako zagolide kuti asonkhanitse swabs monga Orpharyngeal Swabs, nasopharyngeal swab, kapena khungu limawakana. Zimatsimikizira matenda opaka ndi kupezeka kwa ma antigens a ma antigens.
MonkeySox antibody mankhwala, amagwiritsa ntchito njira yagolide yogolide, yokhala ndi zitsanzo kuphatikiza magazi athunthu, ma p lasma, kapena seramu. Imatsimikizira matenda opaka ma antibodies opangidwa ndi munthu kapena thupi la nyama motsutsana ndi kachilomboka kwa MonkesPox.
MonkeyPox Virus Natic acid kuyesa: imagwiritsa ntchito njira yeniyeni yochulukirapo katatu, ndi zitsanzo zomwe zimakhala zotupa. Imatsimikizira matenda opatsirana pozindikira kuti kachilombo ka virus kapena zidutswa zapadera.
Ma Dissealabs 'MonkeyPax Kuyesa
Kuyambira 2015, a Distsealabs 'Monkespox Reagents avomerezedwa pogwiritsa ntchito zitsanzo zenizeni ndipo zatsimikiziridwa chifukwa chodalirika komanso chodalirika. Izi zimaloza mitundu yosiyanasiyana yosiyanasiyana, kupereka makulidwe osiyanasiyana komanso kuchuluka kwa mitundu yosiyanasiyana, ndikuthandizira kuthandizidwa kwambiri ndi kuwunika kwa kachilombo ka konkeyPox komanso kuthandiza bwino pakuwongolera koyenera. Zambiri zokhudzana ndi zida zathu zoyeserera, chonde onaninso: https://www.tessealaxs-
Njira Yoyeserera
Kugwiritsa ntchito swab kuti atole mafinya ku pheture, kusakaniza bwinoBuffer, kenako kugwiritsa ntchito madontho ochepa mu khadi yoyeserera. Zotsatira zake zitha kupezeka pamayendedwe ochepa chabe.
