Ma SAR-COV-2 Onerraction Antibody Oneder Countration Kit (Elisa)
】Kugwiritsa Ntchito】
SAS-COV-2 Onerraction Pulogalamu ya Antibody yolumikizidwa ndi immining immining imminitay (Elisa) kuzindikiritsa ma antibodies onse okwanira ku SARS-Cov-2 mu seramu ya anthu ndi plasma. Zowunikira za Antibont-2 zowononga zitha kugwiritsidwa ntchito ngati chothandizira kuzindikira anthu omwe ali ndi chitetezo chogwiritsira ntchito ku SAST- COV-2, yosonyeza matenda aposachedwa kapena asanafike. Ma SARS-2 Cov-2 omwe amakana antibody dibody sayenera kugwiritsidwa ntchito kuti azindikire matenda owopsa aomwe amawononga ndalama.
】Chiyambi】
Coronavirus matenda amadwala amatulutsa mayankho a antibady. Odwala a serony omwe ali mu covid-19 ndi 50% ndi 100% patsiku 7 ndi 14 positi chisonyezo cholowera, motsatana. Kunena zambiri, kachilombo ka kachilombo ka HIBUS kumadziwika ndi magazi kumadziwika ngati chandamale chofuna kudziwa za antibody kugwiritsa ntchito luso latsopanoli kukuwonetsa kusinthasintha kwamphamvu. Kuyesa kwa Plaque Kupatula (Kuwala) kwakhala mukudziwika kuti ndi muyezo wagolide wofufuza za antibodies. Komabe, chifukwa cha kutsika kwake kotsika komanso zofunika kwambiri pakugwira ntchito, kukwiya sikuthandiza kwa akulu a Serdialnosis ndi kuwunika kwa katemera. Ma SARS-CIB-2 omwe amasakaniza zida za antibody onlict amatengera enyimbo ya enzning imminicler (Elisa), yomwe imatha kuwona njira yolowerera magazi mu chitsanzo cha antibody ya antibody.
】Njira Yoyeserera】
1.Kolekanikirani machubu, alliquot 120μL ya omwe adakonzedwa hace2-hrp yankho.
2.dd 6 μl wa calibrators, zitsanzo zosadziwika, zowongolera zapamwamba mu chubu chilichonse ndikusakaniza bwino.
3.Transfel 100μl iliyonse yosakanizidwa mu sitepe 2 mu malo ofanana microneroplate
3.Coni mbale yokhala ndi mbale yoyala ndi incurute pa 37 ° C kwa mphindi 60.
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5.Tap mbale pa thaulo yamapepala kuti muchotse madzi otsalira mu zitsime mutatsuka.
6.dd 100 μl ya TMB yankho lililonse pachitsime chilichonse ndikusintha mbaleyo mumdima 20 - 25 ° C kwa mphindi 20.
Anthu okwana 50 μl ya kuletsa yankho la chitsime chilichonse kuti aletse zomwe zikuchitika.
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