IVibro Cholerae O139 (VC O139) kunye O1 (VC O1) ComboUvavanyo lusebenzisa ubuchule be-immunochromatography ukuchonga iintlobo ezimbini ezibalulekileyo zebhaktheriya yekholera. Olu vavanyo lubalulekile ekubhaqweni kwekholera kwangethuba, ukuvumela abasemagunyeni kwezempilo ukuba baphumeze ungenelelo olukhawulezileyo. Ukusetyenziswa okusebenzayo kwe-Vibro Cholerae O139 (VC O139) kunye ne-O1 (VC O1) i-Combo iphucula ulawulo lokuqhambuka, ekugqibeleni lunciphisa izinga lokugula kunye nokufa okuhambelana nekholera.
| Unyaka | Amatyala axeliweyo | Ukufa Kwengxelo | Utshintsho Ekufeni |
|---|---|---|---|
| 2023 | 535,321 | 4,000 | + 71% |
Izinto eziphambili zokuThatha
- IVibro Cholerae O139 kunye ne-O1 Combo Testivumela ukubonwa ngokukhawuleza kweentlobo zekholera, okwenza ukuba iimpendulo zempilo yoluntu zikhawuleze.
- Ukuqokelelwa kwesampulu esebenzayo kunye neenkqubo zovavanyo ezifanelekileyo zibalulekile kuxilongo oluchanekileyo lwekholera kunye nolawulo lokuqhambuka.
- Izinto ezintsha zakutsha nje kuvavanyo, olufana novavanyo olukhawulezayo lokuxilonga, luphucula kakhulu isantya sobhaqo kunye nokwandisa iinzame zokucupha ikholera.
Indlela yokusebenza yeVibro Cholerae O139 kunye ne-O1 Combo Test Immunochromatography Technique
Ubuchwephesha boQokelelo lweSampulu
Ukuqokelela okusebenzayo kwesampulu kubalulekile kuvavanyo oluchanekileyo lwekholera. Iingcali zempilo kufuneka zilandele iiprothokholi ezithile zokuqinisekisa imfezeko yeesampulu. Iinkqubo ezicetyiswayo ziquka:
- Iisampulu zesitulo: Qokelela iisampulu ezi-4 ukuya kwezili-10 kwizigulana ekurhaneleka ukuba zinekholera. Ezi sampuli kufuneka zithunyelwe kwilebhu ye-microbiology ukuze kuqinisekiswe, kuchongwe ubunzima, kunye novavanyo lobuzaza bokubulala iintsholongwane.
- Imidiya yezoThutho: Qinisekisa imidiya yezothutho ekhethwayo kunye nelabhoratri. Izinketho zingabandakanya iphepha lokucoca okanye i-Cary-Blair, enceda ukugcina ukusebenza kweesampuli ngexesha lokuthutha.
Iinkqubo zoVavanyo
I-Vibro Cholerae O139(VC O139) kunye ne-O1 (VC O1) Combo Test isebenzisa ubuchule be-immunochromatography obuvumela ukufunyanwa ngokukhawuleza kweentlobo zekholera. Ezi zixhobo zilandelayo kunye nee-reagents zibalulekile xa kuqhutywa uvavanyo:
| Izixhobo / iiReagents | Inkcazo |
|---|---|
| StrongStep® Vibrio cholerae O1/O139 Antigen Combo Rapid Test | Uvavanyo olukhawulezayo olubonakalayo lwe-immunoassay lokukhangela umgangatho we-Vibrio cholerae O1 kunye / okanye i-O139 kwimizekelo ye-fecal yabantu. |
| Anti-Vibrio cholerae O1/O139 amajoni omzimba | I-immobilized kwingingqi yovavanyo ye-membrane yokukhangela. |
| Amasuntswana anemibala | Idityaniswe kwizilwa-buhlungu ukutolika okubonwayo kweziphumo. |
| Umzekelo | Iisampulu zelindle lomntu, ekufuneka zivavanywe ngoko nangoko emva kokuqokelela. |
| Iimeko zokugcina | Gcina ikiti kwi-4-30 ° C, musa ukukhenkcela, kwaye ukhusele ekungcoleni. |
Inkqubo yovavanyo ibandakanya ukusebenzisa isampulu yesitulo kwisixhobo sovavanyo, apho isebenzisana nezilwa-buhlungu. Umgca obonakalayo ubonisa ubukho bebhaktheriya yekholera, evumela ukuxilongwa ngokukhawuleza.
Uvakalelo kunye neNgcaciso
Uvakalelo kunye nokuchaneka kweVibro Cholerae O139 kunye ne-O1 Combo Test ziimetriki ezibalulekileyo zokuvavanya ukusebenza kwayo. Uphononongo lwakutsha nje lweklinikhi luxela la mazinga alandelayo:
| Uhlobo loVavanyo | Uvakalelo | Ukuchaza ngokuthe ngqo |
|---|---|---|
| V. ikholera O139 (iisampulu ezihluziweyo) | 1.5 × 10² CFU/ml | 100% |
| V. ikholera O139 (iisampulu ezingahluzwanga) | Ilog enye isezantsi kunohluzo | 100% |
Ukongeza, uvakalelo oludityanisiweyo kunye neenkcukacha zovavanyo lokuxilongwa ngokukhawuleza lwekholera lubonisa:
| Uhlobo loVavanyo | Uvakalelo oludityanisiweyo | Ingcaciso edityanisiweyo |
|---|---|---|
| Iimvavanyo zokuxilonga ngokukhawuleza kwekholera | 90% (86% ukuya 93%) | 91% (87% ukuya 94%) |
La mazinga aphezulu abonisa ukuba i-Vibro Cholerae O139 (VC O139) kunye ne-O1 (VC O1) iCombo Test Immunochromatography ubuchule bubonelela ngeziphumo ezithembekileyo, okwenza kube sisixhobo esixabisekileyo ekubhaqweni kwekholera kunye nolawulo lokuqhambuka.
Ukubaluleka kwiMpilo yoLuntu
Indima kuLawulo lokuqhambuka
IVibro Cholerae O139 kunye ne-O1 Combo Testidlala indima ebalulekileyo ekulawuleni ukuqhambuka kwekholera. Ukufunyanwa ngokukhawuleza kweentlobo zekholera kuvumela iziphathamandla zempilo ukuba ziphumeze ukungenelela kwangethuba. Olu vavanyo luphakamisa kakhulu isantya kunye nokusebenza kweempendulo zempilo yoluntu.
- Ukwandiswa kovavanyo: Ukuqaliswa kovavanyo olukhawulezayo lokuxilonga (RDTs) kukhokelele ekwandeni kovavanyo lwekholera. Uluntu obekucingwa ukuba alunaso isifo sekholera ngoku lubonisa iimeko ngenxa yokuphuculwa kwamandla okubona.
- Iindleko-Ukusebenza: Ii-RDT zingabizi kakhulu kwaye zichitha ixesha elincinci kuneemvavanyo zasebhubhoratri zendabuko. Oku kusebenzayo kunceda ukuxilongwa ngokukhawuleza kunye nonyango, olubalulekileyo ngexesha loqhambuko.
- Iziphumo zangoku: Uvavanyo olutsha olukhawulezileyo lubonelela ngeziphumo ngemizuzu, ngokukhawuleza kakhulu kunovavanyo lwaselebhu yesintu olunokuthatha iintsuku. Olu tshintsho lukhawulezayo lubalulekile ekuthinteleni ukosuleleka ngakumbi kunye nokuqalisa amaphulo okugonya ngexesha.
Le theyibhile ilandelayo ibonisa ubuntununtunu kunye namazinga aqinisekileyo okubhaqwa kweendlela ezahlukeneyo zokubona ikholera, iqaqambisa iingenelo zeVibro Cholerae O139 kunye ne-O1 Combo Test:
| Indlela | Uvakalelo (%) | Ucalulo (%) | Ireyithi yokuFumana eNgcono (%) |
|---|---|---|---|
| IFAG | 19.9 | Phezulu | 29/146 |
| Inkcubeko eqhelekileyo | 10.3 | Ngasezantsi | 15/146 |
| I-PCR yexesha langempela | 29.5 | Ephezulu | 43/146 |
Izifundo zeMyelo zosetyenziso olusebenzayo
Izifundo zophando zibonisa ukusebenza kweVibro Cholerae O139 kunye ne-O1 Combo Test kwimimandla eyahlukeneyo. Umzekelo, uphando lubonisa umahluko omkhulu kumazinga okumelana namayeza okubulala iintsholongwane phakathi kweVibrio cholerae O139 kunye ne-O1. Iintlobo ze-O1 zihlala zidibaniswa nokuqhambuka okukhulu, ngelixa iintlobo ze-O139 zivame ukudibaniswa neemeko ze-sporadic kunye nokuqhambuka kokutya. Ukuqonda ezi patheni kubalulekile ekulawuleni ubhubhani wekholera, ngakumbi kwiindawo ezisesichengeni njengasemaphandleni e-Bangladesh.
Iimpembelelo zeMpilo yeHlabathi
Umthwalo wehlabathi jikelele wekholera usabalulekile, uchaphazela abantu abamalunga ne-1.3 yebhiliyoni, uninzi lwamatyala lugxile kwi-Afrika ekwi-sub-Sahara nakuMzantsi Asia. Uqhambuko ludla ngokuba luninzi kwaye lube lude, njengoko kungqinwa kumazwe anjengeYemen neHaiti. Iindlela zemveli zegolide zokuxilonga, ezibandakanya inkcubeko yemicrobial kunye nePCR, zifuna ixesha elininzi, abasebenzi abaqeqeshiweyo, kunye neziseko zaselebhu, ezihlala zikhokelela ekulibazisekeni koqhambuko loqinisekiso kunye nokusabela. Le mida inegalelo ekwandeni kokugula nokufa kwaye ithintele uqikelelo oluchanekileyo lomthwalo wekholera, ibeka uxinzelelo olongezelelweyo lwempilo kunye noqoqosho kwimimandla echaphazelekayo.
Kulo mongo, i-immunochromatography-based based diagnostic tests (RDTs) inika indlela yokuguqula. Ngokubona i-Vibrio cholerae O1 kunye ne-O139 antigens ngokusebenzisa i-lateral flow immunoassays, ezi mvavanyo zibonelela ngeziphumo ezisemgangathweni kwimizuzu emi-5, ngaphandle kokufuna ukugcinwa kwekhonkco elibandayo okanye izixhobo ezinzima. Banokulawulwa ngoqeqesho oluncinci kwindawo yokunyamekela, okwenza ukuba baxabiseke ngokukodwa kwiindawo ezikude kunye nezixhobo eziphantsi. Nangona kungajoliswanga ukuxilongwa kwesigulane esicacileyo, i-RDT inexabiso eliphezulu elibi lokuxela kwangaphambili, ukunciphisa imfuno yovavanyo oluqinisekisayo kwiindawo ezisezantsi. Esona sicelo sabo siphambili silele kucupho lwe-epidemiological, apho isantya sabo kunye nokusebenza kakuhle kweendleko kuvumela ukubonwa kwangethuba, ukubekwa esweni ngcono kweendlela ze-spatiotemporal, kunye nokusasazwa okusebenzayo kongenelelo olunje ngogonyo lwekholera yomlomo (OCVs) kunye namanyathelo ococeko-ingakumbi abaluleke kakhulu ngokunikezelwa kwe-OCV elinganiselweyo yangoku.
Iimpembelelo zokwamkelwa kwe-immunochromatography zifikelela kude: uphononongo oluphuculweyo lwexesha lokwenyani luphucula ukuchaneka kwengqikelelo kunye nokwandisa impendulo yokuqhambuka; ukulinganisa iinkcazo zamatyala kumazwe ngamazwe kuba yinto enokwenzeka ngakumbi ngovavanyo olukhawulezayo oludityanisiweyo; kunye nemijelo yedatha ephumayo inokudityaniswa kunye nobukrelekrele bokwenziwa ukuze kuhlalutywe nzulu ukuhanjiswa kwamandla. Ekugqibeleni, olu tshintsho luyimfuneko ekuqhubeleni phambili ulawulo lwekholera yehlabathi, ukunciphisa ukufa okunokuthinteleka, kunye nokunciphisa iimpembelelo zempilo kunye nezoqoqosho kubantu abasesichengeni.
IVibro Cholerae O139 kunye ne-O1 Combo Testidlala indima ebalulekileyo ekubhaqweni kwekholera. Ichonga ngokuthembekileyo iintlobo zekholera, ivumela iimpendulo ezikhawulezayo zempilo yoluntu. Ngovakalelo lokubhaqa nje ezimbalwa njenge 103 iiseli zeV. ikholera, olu vavanyo lubonisa ukuba luyimfuneko kulawulo loqhambuko.
Ukwandisa ulwazi kunye nokusetyenziswa kolu vavanyo phakathi kwababoneleli bezempilo kubalulekile. Le theyibhile ilandelayo iqaqambisa ukuxhaphaka kunye nokuxhathisa amayeza okubulala iintsholongwane kwi-cholera serogroups:
| Serogroup | Ukuxhaphaka (%) | Ukunyangwa kweAntibiotic (%) |
|---|---|---|
| O1 | Phezulu | 70% (cefotaxime), 62.4% (trimethoprim-sulfamethoxazole), 56.8% (ampicillin) |
| O139 | Phakathi | N / A |
Abasemagunyeni kwezempilo kufuneka balubeke phambili olu vavanyo ukunyusa iinzame zokulawula ikholera kwihlabathi jikelele.
FAQ
Yintoni eyona njongo iphambili yoVavanyo lweVibro Cholerae O139 kunye ne-O1 Combo?
Uvavanyo luchonga ngokukhawuleza iintlobo zekholera, nto leyo evumela ungenelelo lwezempilo loluntu ngexesha elifanelekileyo.
Kuthatha ixesha elingakanani ukufumana iziphumo kuVavanyo lweCombo?
Funda iziphumo ngemizuzu emi-5. Musa ukutolika umphumo emva kwemizuzu eyi-10.
Ewe, uvavanyo lunokubona ngaxeshanye zombini iVibrio cholerae O1 kunye ne-O139 iintlobo kwisampulu enye.
Ixesha lokuposa: Sep-05-2025