Testsealabs Chikungunya IgM Test

Chikungunya IgM Test

1. Uhlalutyi ekujoliswe kulo: Olu vavanyo luchonga ngokukodwa izilwa-buhlungu zeklasi ze-IgM eziveliswa yi-immune system ekuphenduleni usulelo lwe-Chikungunya virus. Izilwa-buhlungu ze-IgM ngokuqhelekileyo zezokuqala ukuvela ngexesha losulelo oluqatha, luqhele ukubonwa phakathi kweentsuku ezi-3-7 emva kokuqala kweempawu kwaye ziqhubeke iiveki ezininzi ukuya kwiinyanga. Ukufumanisa kwabo ke ngoko kuluphawu olubalulekileyo losulelo lwamva nje okanye oluqatha lwe-CHIKV.
2. Ukuhambelana kweSpecimen: Uvavanyo luqinisekisiwe ukuba lusetyenziswe kunye neentlobo ezininzi zeesampulu, ukubonelela ngokuguquguquka kwezicwangciso ezahlukeneyo zempilo:

• Igazi Elipheleleyo (Ifingerstick okanye iVenipuncture): Ivumela ukhathalelo olukhawulezileyo okanye uvavanyo olusondeleyo lwesigulane ngaphandle kwesidingo sokwenziwa kwesampulu enzima.
• ISerum: Uhlobo lwesampulu esemgangathweni yegolide yokufunyanwa kwe-antibody kwisethingi zaselabhoratri.
• I-Plasma: Ibonelela ngenye indlela kwiserum, ehlala ifumaneka lula kwiilebhu zeklinikhi.

3. Ukusetyenziswa okucetywayo kunye neXabiso lokuHlola: Eyona njongo iphambili yolu vavanyo kukunceda abaqeqeshi bezempilo ekuxilongeni usulelo olubukhali lweChikungunya virus. Isiphumo esihle se-IgM, ngakumbi xa sihambelana neempawu zeklinikhi (umkhuhlane ophezulu ngokukhawuleza, intlungu edibeneyo, i-rash, intloko, njl. Ibaluleke kakhulu kwisigaba sokuqala sesigulo xa izilwa-buhlungu ze-IgG zisenokungabonakali.
4. UmGaqo weTekhnoloji: Ngokusekwe kwitekhnoloji ye-chromatographic immunoassay esecaleni:

• I-Colloidal Gold Conjugate: I-strip yokuvavanya iqulethe i-pad ene-antigen ye-CHIKV edityaniswe kwiincinci zegolide ze-colloidal.
• Ukuqukuqela kwesampulu: Xa isampulu (igazi, i-serum, okanye iplasma) isetyenziswa, ifuduka ngokwekhromatografi ecaleni komcu.
• I-Antibody Capture: Ukuba i-CHIKV-specific IgM antibodies ikhona kwisampulu, iya kubophezela kwii-antigens ze-CHIKV zegolide, zenze i-antigen-antibody complex.
• Test Line Capture: Le ngqungquthela iyaqhubeka ihamba kwaye ibanjwe yi-anti-human IgM antibodies immobilized kwi-Test (T) yomgca wengingqi, okubangelwa umgca ombala obonakalayo.
• Umgca wokulawula: Umgca woLawulo (C), oqulethe i-antibodies ebopha i-conjugate kungakhathaliseki ukuba i-CHIKV antibodies, kufuneka ihlale ibonakala iqinisekisa ukuba uvavanyo lusebenze ngokuchanekileyo kwaye isampuli ifuduke ngokufanelekileyo.

5. Iziphumo eziKhawulezayo: Uvavanyo lubonelela ngesiphumo esibonakalayo, esisemgangathweni (Esilungileyo/embi) ngokwesiqhelo kwimizuzu eyi-10 ukuya kwengama-20, siququzelela ukuthathwa kwezigqibo zeklinikhi ngokukhawuleza.
6. Ukusebenziseka ngokulula: Yenzelwe ukulula, kufuna uqeqesho oluncinci kwaye akukho sixhobo sikhethekileyo sokutolika iziphumo, iyenza ifaneleke kwizicwangciso ezahlukeneyo ezibandakanya iiklinikhi, iilabhoratri, kunye nokusetyenziswa kwendawo ngexesha lokuqhambuka.
7. Iingqwalasela ezibalulekileyo:

• Umgangatho: Olu luvavanyo lokuhlola olunika impendulo ethi Ewe/Hayi yobukho be-IgM antibodies, hayi ubungakanani (titer).
• Unxulumano lwezonyango: Iziphumo kufuneka zitolikwe kunye nembali yekliniki yesigulana, iimpawu, umngcipheko wokuvezwa, kunye nezinye izinto ezifunyaniswe kwilebhu. Izilwa-buhlungu ze-IgM ngamanye amaxesha zinokuzingisa okanye zihlangane neentsholongwane ezinxulumeneyo (umzekelo, i-O'nyong-nyong, iMayaro), ezinokuthi zikhokelele kwizinto ezingeyonyani. Kwelinye icala, ukuvavanywa kwangoko kusulelo (phambi kokuba i-IgM inyuke iye kumanqanaba abonakalayo) inokuvelisa izinto ezingezizo ezingezizo.
• Uvavanyo olongezelelweyo: Kwezinye ii-algorithms zokuxilonga, i-IgM efanelekileyo inokulandelwa kunye neemvavanyo ezithile (ezifana ne-Plaque Reduction Neutralization Test - PRNT) yokuqinisekisa, okanye uvavanyo lwe-IgG oludityanisiweyo (kwiisampuli ezibukhali kunye ne-convalescent) zingasetyenziselwa ukubonisa i-seroconversion.