Testsealabs Ọrịa Ọrịa ịba Ag pf/pv Ule Tri-line

Nnwale Ọrịa Testsealabs Ọrịa ịba Ag pf/pv Oyiyi egosipụtara n'ahịrị atọ

Nkọwa dị mkpirikpi:

The Malaria Ag Pf/Pv Tri-line Cassette bụ immunoassay ngwa ngwa chromatographic maka nchọpụta qualitative nke plasmodium falciparum histidinerich protein-II (HRP-II) na plasmodiumvivax lactate.dehydrogenase (LDH) n'ime ọbara dum iji nyere aka na nyocha nke ịba (Pf / Pv).

Nsonaazụ ngwa ngwa: Lab-nke ziri ezi na nkeji	Nkọwapụta nke ụlọ nyocha: Kwesịrị ntụkwasị obi na ntụkwasị obi
Nwalee ebe ọ bụla: Enweghị nleta ụlọ nyocha achọrọ	Ogo enwetara: 13485, CE, Mdsap kwadoro
Dị Mfe & Nhazi: Ọ dị mfe iji, nsogbu efu	Ọdịmma kacha mma: nwalee nke ọma n'ụlọ

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Nkọwa ngwaahịa:

Ụdị nlele:
- Ọbara dum (mkpịsị aka mkpịsị aka ma ọ bụ ọbara venipuncture).
Oge nchọpụta:
- 15-20 nkeji(a ga-atụgharịrị nsonaazụ ya n'ime nkeji 20; nsonaazụ mgbe oge a gachara abaghị uru).
Mmetụta na nkọwapụta:
- Mmetụta:Na-emekarị> 90% maka ịchọpụta ma ọrịa Pf na Pv.
- Nkọwapụta:Na-emekarị> 95% maka nchọpụta Pf na Pv.
Ọnọdụ Nchekwa:
- Chekwaa n'etiti4°C na 30°C, pụọ na ìhè anyanwụ kpọmkwem.
- Ajụla oyi.
- Ndụ nchekwa na-abụkarị site naỌnwa 12 ruo 24, dabere na ntuziaka onye nrụpụta.
Nkọwapụta nsonaazụ:
- Nsonaazụ dị mma:
  - Ahịrị atọ a na-ahụ anya:
    1. C (Control) ahịrị(gosi na ule ahụ dị irè).
    2. Ahịrị Pf(ọ bụrụ na achọpụtara antigens Plasmodium falciparum).
    3. Ahịrị pv(ọ bụrụ na achọpụtara antigens Plasmodium vivax).
    - Ọnụnọ nke ahịrị Pf na/ma ọ bụ Pv na-egosi ọrịa na ụdị ịba dị iche iche.

Ụkpụrụ:

Nyocha Immunochromatographic:
Kaseti ule nwere enweghị ike imegharị yaọgwụ mgbochi monoclonalkpọmkwem maka Plasmodium antigens (dịka ọmụmaatụ,HRP-2maka Pf napLDHmaka Pv).

Mgbe etinyere ọbara n'ule, ma ọ bụrụantigens ịbadị ugbu a, ha ga-ejikọta na ọgwụ mgbochi ọla edo na-ejikọta na ihe nlele ahụ, nke ga-agafe akpụkpọ ahụ ule site na capillary edinam.
Ọ bụrụ naPlasmodium falciparumAchọpụtara antigen, eriri agba ga-etolite naAhịrị Pf.
Ọ bụrụ naPlasmodium vivaxAchọpụtara antigen, eriri agba ga-etolite naAhịrị pv.
NkeAhịrị njikwa (C)na-eme ka ule ahụ na-arụ ọrụ nke ọma ma gosipụta izi ezi nke ule ahụ.

Ihe mejupụtara:

Ihe mejupụtara	Ọnụ ego	Nkọwapụta
IFU	1	/
Nwalee cassette	25	Akpa foil ọ bụla emechiri emechi nwere otu ngwaọrụ nnwale na otu ihe eji eme ihe
mmịpụta diluent	500μL * 1 Tube * 25	Ihe nchekwa Tris-Cl, NaCl, NP 40, ProClin 300
Ntụpọ dropper	1	/
Swab	/	/

Usoro nnwale:


1. Saa aka gi	2. Lelee ihe dị n'ime ngwa ahụ tupu ịnwale, gụnyere ntinye ngwugwu, kaseti ule, ihe nchekwa, swab.	3. Tinye tube mmịpụta na ebe a na-arụ ọrụ.	4.Kpechapụ ihe mkpuchi aluminom si n'elu nke tube mmịpụta nke nwere ihe ntinye ihe.

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5. Jiri nlezianya wepụ swab na-emetụghị ọnụ ọnụ ahụ. Tinye ọnụ ọnụ nke swab 2 ruo 3 cm n'ime imi ziri ezi. Rịba ama ebe mgbawa nke imi imi. Ị nwere ike inwe mmetụta nke a na mkpịsị aka gị mgbe ị na-etinye imi imi ma ọ bụ lelee ya na mimnor. Na-ete ime ime imi na mmegharị okirikiri ugboro 5 maka opekata mpe 15 sekọnd, Ugbu a were otu imi imi ma tinye ya n'oghere imi ọzọ. Biko jiri ihe nlele were mee nnwale ahụ ozugbo ma emela ya
hapụ ya ka ọ kwụ.

6. Tinye swab na tube mmịpụta. Gbanwee swab maka ihe dị ka 10 sekọnd, gbanwee swab megide tube nke na-emepụta ihe, na-agbanye isi nke swab n'ime ime tube ahụ ka ị na-agbanye n'akụkụ akụkụ nke tube iji hapụ mmiri mmiri dị ka o kwere mee site na swab.


7. Wepụ swab na ngwugwu na-emetụghị ihe mgbochi ahụ aka.	8.Mix nke ọma site n'ịgbanye ala nke tube ahụ. Tinye 3 tụlee nke nlele ahụ n'ụzọ ziri ezi n'ime ihe nlele nke ọma nke cassette ule. Gụọ ihe si na ya pụta mgbe minit 15 gasịrị. Rịba ama: Gụọ nsonaazụ n'ime nkeji 20. Ma ọ bụghị ya, a na-akwado arịrịọ maka ule.

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